Hi Loralei, I will see If I can find someone with a sliding microtome. Are you using OCT to block the 50 um section? How do you ensure the section stays flat while blocking/freezing? Thank you for your reply ! E-van
From: Loralei Dewe [mailto:lld...@gmail.com] Sent: Friday, October 06, 2017 6:13 PM To: Dessasau III, Evan <edde...@emory.edu> Subject: Re: [Histonet] FW: cutting a 5um frozen section from a 50um section of brain I would use a sliding microtome and freeze the 50 um sections solid with dry ice. I used to do sections of mouse brain that way. Loralei On Oct 6, 2017 9:10 AM, "Dessasau III, Evan via Histonet" <histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>> wrote: Hi Histonet , I have been trying to cut a FLAT 4 to 10 um frozen section from 50um sections of brain. Every time I think I have the tissue flat the sections are never in the same plane. I found a wonderful book in pdf format(A Practical Guide to Frozen Section Techniques, Stephen R. Peters) online with lots of wonderful tips but I'm having no luck implementing the tips. Has anyone ever tried this? Any help is GREATLY appreciated. Thank you, E-van E-van D. Dessasau, III, HTL(ASCP)cm Supervisor, Histology Division of Pathology Emory University Yerkes NPRC Main Center Rm. 2122 954 Gatewood Rd. Atlanta, GA. 30329 (404)727-7744<tel:%28404%29727-7744> lab (404) 727-7902<tel:%28404%29%20727-7902> office ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
