Hey Joe, All the specimens come to the lab fresh. Instead of the Cytospin collection fluid we have been using thin prep preservcyt solution to resuspend, which is mostly methyl alcohol. Do you think that would have some influence to the washing problem. We are staining with the PAP stain. Thanks for your input let me know if you have anymore suggestions. Nina ________________________________________ From: Joe W. Walker, Jr. <jwwal...@rrmc.org> Sent: Thursday, November 2, 2017 12:03 PM To: Nina J. Rich; histonet@lists.utsouthwestern.edu Subject: RE: cytospin prep
WARNING: This email originated outside of our company Beaufort Memorial DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe Hi Nina, How are the cytology specimens collected? This will play a role in how you process them for evaluation on the Cytospin machine. We do not utilize the mega funnels. However, cytology specimens should be centrifuge first to remove any supernatant. Then equal parts of the Cytospin collection fluid should be added to the cell button and the cell button should be resuspended. Once resuspended, the amount of specimen that you put into the mega funnel chamber will vary depending on the cellularity of the cytology specimen. Large cell buttons might require less while small buttons will require more. If you over load the chamber, you will experience the cell spot/square falling off the slide when staining. This assumes that you are Pap staining your slides, hence the use of the Cytospin collection fluid. If you are making airdried specimens for Romanowsky stains, if you over load the chamber, you will have the same result of the spot falling of the slide. The manufacturer should provide max volume limits for the mega funnels but again a very cellular specimen will create issues. This has been our experience with cytospins. Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P 802.747.1790 F 802.747.6525 joewal...@rrmc.org, www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Our Commitment to our Community: We Listen, We Respect, We Care . . . Always. Joint Commission Accredited | Best Regional Hospital: U.S. News & World Report | Leapfrog Hospital Safety A Rating ANCC Magnet Hospital Designation(r) | Healthgrades: Excellence Award for Patient Safety | Healthgrades: Outstanding Patient Experience Award -----Original Message----- From: Nina J. Rich via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, November 02, 2017 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cytospin prep Good morning, For all those that have to do cytology cytospins. We are using the cytospin 4 with the large square mega funnels. We have a problem with the specimen always washing off the slide. Is there anyone who can send me their processing procedure for this type of funnel, to include whether or not you centrifuge the specimen first. The small circular funnels do not wash. Any input would greatly be appreciated. Jean _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
