This used to be a common problem years ago. It is due to crud buildup on
the metal. Boil them with TCP for half an hour, then thoroughly wash
them in cold water. Coat them with a VERY light smear of glycerol before
you use them, preferably each time. That should help.
Bryan Llewellyn.
Diane Satterfield via Histonet wrote:
We are using large metal molds to embed mouse brains. We are having a hard
time getting to block out of the molds, the paraffin blocks are sticking.
Sometimes they are coming out cracked. Sometimes the cassette comes off the
paraffin block. Any idea why this is happening? Any advice on how to fix this
problem?
Diane L. Satterfield, BS
Manager Brain Tumor BioRepository
Research Program Leader
Duke University Medical Center
Brain Tumor Center Biorepository and Database
diane.satterfi...@duke.edu<mailto:diane.satterfi...@duke.edu>
office 919-684-4642
pager 919-970-7328
fax 919-684-4975
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