Good Morning Kelly, I work in a research institute that works with murine samples; In our laboratory we have adopted two descaling protocols: one with ion exchange resins/acid solution while the other uses EDTA. The EDTA descaling system is slower but keeps the nucleic acids much better. We use an 14% solution of tetrasodium EDTA in water buffered to pH 7.4-7.6 with acetic acid, the solution is left for several days (from 5 to 10 depending on the density and size of the bone) with daily solution changes.
Have a good work Stefano Dott. Stefano Mantero Human Genome Laboratory CNR-IRGB c/o Humanitas Research Hospital Via Rita Levi Montalcini (Ex Via Dainese) 20090 Pieve Emanuele (MI) Ph. +39 02 8224 5164 (desk) Ph. +39 02 8224 5177 (lab) Fax +39 02 8224 5191 ------------------------------------------ DAI IL TUO 5x1000 ALLA RICERCA HUMANITAS. Codice fiscale 10125410158 (Ricerca sanitaria) http://www.humanitas.it/5x1000 ------------------------------------------ Nota di riservatezza. Il presente messaggio, corredato dei relativi allegati, contiene informazioni da considerarsi strettamente riservate, ed è destinato esclusivamente al destinatario sopra indicato, il quale è l'unico autorizzato ad usarlo, copiarlo e, sotto la propria responsabilità, diffonderlo. Chiunque ricevesse questo messaggio per errore o comunque lo leggesse senza esserne legittimato è avvertito che trattenerlo, copiarlo, divulgarlo, distribuirlo a persone diverse dal destinatario è severamente proibito, ed è pregato di rinviarlo immediatamente al mittente distruggendone l'originale. Grazie Confidentiality Notice. This message, together with its annexes, contains information to be deemed strictly confidential and is destined only to the addressee(s) identified above who only may use, copy and, under his/their responsibility, further disseminate it. If anyone received this message by mistake or reads it without entitlement is forewarned that keeping, copying, disseminating or distributing this message to persons other than the addressee(s) is strictly forbidden and is asked to transmit it immediately to the sender and to erase the original message received. Thank You -----Messaggio originale----- Da: Pairan, Kelly via Histonet [mailto:[email protected]] Inviato: venerdì 10 novembre 2017 12:39 A: [email protected] Oggetto: [Histonet] EDTA decalcification tissue issues Good Morning, Recently, our lab has been working on validating an EDTA method of decalcification. When we ran the IHC's on the decalcified bone block, the majority of the tissue lifted off the slide. We use the Leica Bonds for our IHC staining. Does anyone else have a hard time getting EDTA decalcified tissue to stay on positively charged slides during IHC runs? Do you have a trick you use? We really did not have this big of a problem when we were decalcifying with Formical or Nitrical (big bones only). Thanks for your help, ?Kelly Pairan, HT(ASCP), QIHC(ASCP) _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
