Hello all, and especially those that offered advice. Thanks for the advice. I will be experimenting with the options today and tomorrow.
Thanks for being so generous with information. Kind regards On Sun, Feb 4, 2018 at 12:00 PM, <histonet-requ...@lists.utsouthwestern.edu> wrote: > Date: Sat, 3 Feb 2018 13:50:11 -0500 > From: Bob Richmond <rsrichm...@gmail.com> > > Bryan Llewellyn refers you to > http://stainsfile.info/StainsFile/stain/oversight/romanowsky.htm > > That method prescribes neutral buffered formalin fixation. Us old-timers > preferred Zenker fixation, which you can't do today on account of the > mercury in it. You may have to try different fixatives. > > The differentiation under microscopic control is really necessary - when I > was a resident at Cornell Medical Center in NYC fifty years ago, the > histotechs would push the residents off the double-headed microscopes when > they sat down to differentiate the bone marrow biopsy section Giemsa > stains. > > The old-timers used a 10% solution of colophonium rosin (almost pure > abietic acid) in alcohol, instead of acetic acid, to differentiate the > stain. > > You buy Giemsa or Wright or Romanovsky stain already dissolved in absolute > alcohol. You have to be a fanatic about not contaminating your stock bottle > with the tiniest trace of water. > > Bob Richmond > Samurai Pathologist > Maryville TN > -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. *Please note: I don't usually check my email over the weekend. * _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet