Good Morning HistoNet, Would anyone be willing to share what they use for control tissue for MMR testing? Finding negative expressing tumor is becoming more difficult for us to find and we're wondering if there is an alternate negative tissue that would be acceptable.
NordiQC recommends using Tonsil as a positive tissue control and Colon Adenocarcinoma that has a loss of expression as a negative tissue control for all four antibodies (MLH1, MSH2, MSH6, PMS2). Stromal cells in the negative tissue control should have positive nuclear staining and serve as a positive internal control. NordiQC doesn't have any recommendations for alternative tissue to use as a negative control. Anyone doing anything differently? Thanks, Scott Scott A. Lindrud, MLS(ASCP)CM CTCM Histopathology Technical Specialist/Cytotechnologist Rice Memorial Hospital 301 Becker Ave SW Willmar, MN 56201 WP(320)231-4406 Fax(320)231-4861 scott.lind...@rice.willmar.mn.us ________________________________ "This e-mail transmission is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution violates confidentiality and privacy laws and is prohibited. If you are not the intended recipient, please notify the sender immediately and delete all copies of the message. Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
