I'm certified in EM. My suggestion would be to use SEM as opposed to TEM. The scanning electron microscope (SEM) can also be useful to reveal morphological features of isolated organisms as well as for diagnosis. There are numerous articles citing this technique. I believe the UC Berkley has an SEM in their lab. I hope this helps. Terri
Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 1. Prosthetic lens processing, cutting? (Morken, Timothy) Message: 1 Date: Thu, 8 Mar 2018 19:30:35 +0000 From: "Morken, Timothy" <timothy.mor...@ucsf.edu> To: Histonet <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Prosthetic lens processing, cutting? Hi all, Does anyone have any experience with embedding an acrylic prosthetic lens for EM, or any other way ? We have a case where they wanted EM on a lens to identify a specific bacteria. We have never done it and it would take some work to figure it out. Also, I told them that unless there is some morphologic marker for the bacteria, EM would not help much identifying it. Any ideas? Any labs that handle this kind of thing? They are willing to send it out. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center *********************** --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet