Hello Histonet, I am just starting a project using LCM for RNA extraction. So far, our tissues haven't stayed on the slides very well. We are using the Acrturus LCM staining kit.
We've taken meniscus at 10, 20, 30, and 40uM sections and started the staining process with (10mins at room temp) and without drying. We want to make sure not to degrade any RNA. Some tissues stay on the slides through to the xylenes, but when we air dry for 5mins, they begin to shrink and pick up from the slides. Our next trial will be with thinner sections, and also trying a short PFA fixation for 10mins. Do you have any suggestions for keeping these sections on the slides and not causing issues with RNA? Thank you for any advice you may have. Sincerely, Merissa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet