Hello Nancy, we have done some histology on sponges and tried paraffin and resin embedding. Due to the spicules and also biofilms and sand etc. the best results could be reached with resin embedding in technovit 7100 or better 9100 or 7200 with cutting grinding techniques. Sectioning of 9100 or 7100 is possible, but the spicules can cause damages in the sections, so best results can be reached with cutting grinding.
There are several trichrome and PAS stainings which give nice results, but we have learned that individual testings have to be done, because the sponge tissues are very different in their preservation and stainings abilities. For fixation you should use formaline, but other fixative are also fine, depending on what you want to see. Ethanol does not give good results and picric acid might cause problems during polymerization. So I hope this helps you a little bit. With best regards Michael > Am 27.04.2018 um 17:08 schrieb Thomas, Nancy via Histonet > <histonet@lists.utsouthwestern.edu>: > > I would like to hear from anyone with experience in working with the > pineapple sponge and other similar types. A future project will include > processing, sectioning and staining of the samples in order to identify them > by viewing the arrangement of the spicules. I am wondering if cryo, paraffin > or plastic would be the best method? How much fixation is necessary? If > anyone has working protocols they would share, I would be so thankful. > > > Nancy Thomas > > Senior Lab Manager, Histology Core > > Stowers Institute for Medical Research > > 1000 E. 50th Street > > Kansas City, MO 64110 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************************************ MORPHISTO GmbH PD Dr. phil. nat. Michael Gudo Weismüllerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.g...@morphisto.de <mailto:i...@morphisto.de> Internet: http://www.morphisto.de/ <http://www.morphisto.de/> Vertretungsberechtigter Geschäftsführer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gemäß § 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
