Hi Merissa, As far as I am aware, insufficient paraffin infiltration would only affect sectioning. The epitopes that we we are attempting to stain with IHC are affected by pre-analytic factors such as fixation, cold ischemic time and perhaps heat (too much) but plus or minus wax should not be an issue if you are able to obtain good sections. Have you stained these poorly infiltrated specimens with H&E? Are you certain you have good sections with all tissue components represented? That would be my main concern. Greg
-- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet