Dear Jessica, fixation should be at least 48 hours, however it is not problematic if the tissues stay longer in these solutions. Important point is, to remove the picric acid, which is a component of the Bouin solution, with several steps of 70 - 80 % of ethanol, so after fixation go directly into 70 - 80 % ethanol, and change the ethanol several times, until the ethanols stay clear. To improve this process, you can add a few drops of ammoniak.
Best regards Michael > Am 24.05.2018 um 23:49 schrieb Jessica Riggleman > <jriggle...@globusmedical.com>: > > Michael, > Thank you so much. How long do you live it in the fixation > solution/alcohol/etc for typically? > > Thank You, > Jessica > > <image9ca84c.PNG> <http://www.globusmedical.com/> Jessica Riggleman | > Research Associate > > Globus Medical, Inc. > Valley Forge Business Center > 2560 General Armistead Avenue | Audubon, PA 19403 > Ph: (610) 930-1800 ext. 2583 | Fax: > An ISO 13485 Registered Company > > website <http://www.globusmedical.com/> | map > <http://www.google.com/maps/place/2560+General+Armistead+Ave,+Eagleville,+PA+19403/@40.1200296,-75.4002192,17z/data=!3m1!4b1!4m2!3m1!1s0x89c696854623b3bb:0xbe9c99708978a5e6> > | email <mailto:jriggle...@globusmedical.com> > > Confidentiality Note: This email is confidential and intended solely for the > use of the individual to whom it is addressed. If you are not the intended > recipient, be advised that you have received this email in error and that any > use, dissemination, forwarding, printing, or copying of this email is > strictly prohibited. If you have received this email in error please contact > the sender. Any views or opinions presented are solely those of the author > and do not necessarily represent those of Globus Medical, Inc. Although this > email and any attachments are believed to be free of any virus or other > defects which might affect any computer or IT system into which they are > received, no responsibility is accepted by Globus Medical, Inc. for any loss > or damage arising in any way from the receipt or use thereof. > From: Michael Gudo (Morphisto GmbH) [mailto:michael.g...@morphisto.de > <mailto:michael.g...@morphisto.de>] > Sent: Thursday, May 24, 2018 3:59 PM > To: Jessica Riggleman <jriggle...@globusmedical.com > <mailto:jriggle...@globusmedical.com>> > Cc: histonet@lists.utsouthwestern.edu > <mailto:histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Umbilical Cord Embedding > > Dear Jessica, > > we have done embedding in paraffine and the results are quite good. The > critical point is fixation. Due to the high proportion of water in the > tissue, the best fixation is possible with Bouin-solution or - if you want to > do IHC stainings, Zamboni-fixation (which is a variant of Bouin with a > buffered pH value). > > With trichromatic or polychromatic stainings you will get very colorful > results. > > Best regards > Michael > > > > Am 24.05.2018 um 21:01 schrieb Jessica Riggleman via Histonet > <histonet@lists.utsouthwestern.edu > <mailto:histonet@lists.utsouthwestern.edu>>: > > Hello, > Does anyone have any experience embedding umbilical cord tissue? Preferably > paraffin but we may try plastic as well. Any help/protocols/etc. would be > appreciated. > > Thank You, > Jessica > > > _____________________________________________________________________ > > Jessica Riggleman | Research Associate > > Globus Medical, Inc. > Valley Forge Business Center > 2560 General Armistead Avenue | Audubon, PA 19403 > Ph: (610) 930-1800 ext. 2583 | Fax: > > Confidentiality Note: This email is confidential and intended solely for the > use of the individual to whom it is addressed. If you are not the intended > recipient, be advised that you have received this email in error and that any > use, dissemination, forwarding, printing, or copying of this email is > strictly prohibited. If you have received this email in error please contact > the sender. Any views or opinions presented are solely those of the author > and do not necessarily represent those of Globus Medical, Inc. Although this > email and any attachments are believed to be free of any virus or other > defects which might affect any computer or IT system into which they are > received, no responsibility is accepted by Globus Medical, Inc. for any loss > or damage arising in any way from the receipt or use thereof. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu <mailto:Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > <http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > ************************************************************************************************ > MORPHISTO GmbH > PD Dr. phil. nat. Michael Gudo > Weismüllerstr. 45 > 60314 Frankfurt am Main > Telefon: 069 / 400 3019 - 62 > Telefax: 069 / 400 3019 - 64 > > E-Mail: michael.g...@morphisto.de <mailto:i...@morphisto.de> > Internet: http://www.morphisto.de/ <http://www.morphisto.de/> > > Vertretungsberechtigter Geschäftsführer: Dr. Michael Gudo > > Registergericht: Amtsgericht Frankfurt > Registernummer: HRB 74954 > Umsatzsteuer-Identifikationsnummer gemäß § 27 a Umsatzsteuergesetz: > DE243397199 > ************************************************************************************************ > Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder > dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der > unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe > des Inhaltes der Email nicht gestattet ist. 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Michael Gudo Weismüllerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.g...@morphisto.de <mailto:i...@morphisto.de> Internet: http://www.morphisto.de/ <http://www.morphisto.de/> Vertretungsberechtigter Geschäftsführer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gemäß § 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet