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Subject: Histonet Digest, Vol 175, Issue 15
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Today's Topics:
1. Staining Protocol for Chromosome Counting in Plants
(Bharti Parihar)
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Message: 1
Date: Fri, 15 Jun 2018 15:09:00 -0700
From: Bharti Parihar <bhartolog...@gmail.com>
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Staining Protocol for Chromosome Counting in
Plants
Message-ID:
<CAJxUEriJH7rNRJ7pPPEP=8mjxk8pck6ZxuO=8j2w11yhf_n...@mail.gmail.com>
Content-Type: text/plain; charset="UTF-8"
Hi ya'll. If there are any individuals out there that have done karyotyping, I
need to try a protocol to get a chromosome count on a desert plant. We have
germinated seeds with long roots, so sample size shouldn't be an issue. Tried a
Orcein stain, but the results are quite lacking and it may not be the right
stain for this plant. Gonna do a bit of modification with the fixative to
specimen ratio to see if that helps, but it's possible this just isn't the
right stain. After all, the stain ordered is for Arabidopsis, which is not our
research plant. This is my first time doing plant histo, I'm used to human
tissue, so I really don't know which stain to try. Anyone with any help/advice
to offer, I would immensely appreciate it!
--
Bharti Parihar, HT (ASCP)CM
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End of Histonet Digest, Vol 175, Issue 15
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