Hey Jennifer, Thanks for your help. I have two questions: 1. What is the point of decalcifying if they are in plastic? 2. Did you see the counterstain? Right now only the bone is staining. How long did you leave these in the counterstain for? Thanks! Jessica
From: Mac Donald, Jennifer [mailto:jmacdon...@mtsac.edu] Sent: Tuesday, July 10, 2018 4:07 PM To: Jessica Riggleman <jriggle...@globusmedical.com>; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] New Bone Stain I used to do it on plastic sections of in-de-calcified bone biopsies. It’s a silver reaction. Get Outlook for iOS _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. ________________________________________ From: Jessica Riggleman <jriggle...@globusmedical.com> Sent: Tuesday, July 10, 2018 1:01 PM To: Mac Donald, Jennifer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New Bone Stain Thank You, Have you ever tried this on thick histology sections? I have a titanium implant in this section, so thin is impossible. Thank You, Jessica ________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: An ISO 13485 Registered Company Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. From: Mac Donald, Jennifer [mailto:jmacdon...@mtsac.edu] Sent: Monday, July 09, 2018 6:09 PM To: Jessica Riggleman <jriggle...@globusmedical.com>; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] New Bone Stain von Kossa will stain the mineralized bone black and a basic fuschin counter stain the osteoid will stain deep pink. Get Outlook for iOS ________________________________________ From: Jessica Riggleman via Histonet <histonet@lists.utsouthwestern.edu> Sent: Monday, July 9, 2018 1:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Bone Stain Hello, I am trying to assess new bone vs. old bone in an animal model. Any suggestions on thick section stains? I have heard Van Gieson's? Thank You, Jessica _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
