HI Mary Ann,

If you are referring to the Pap stain for Pap tests or for Non-Gyn specimens 
including FNA, you will need to verify daily that the technical quality of the 
slides.  This evaluation should include any preparation for the day, including 
liquid based, cytospins, direct smears, and cell blocks.  The slides should be 
evaluated to ensure the presentation of the cells, including the staining 
characteristics are appropriate for the nuclei and cytoplasm of the cells in 
question.  In our lab, we evaluate a slide from each staining run.  If we run 
only Pap tests, then a slide is randomly pulled from the run and evaluated. For 
non-gyn specimens, we apply the same technique if those specimens types were 
run on any day.  For non-gyn specimens, we also evaluate the Romanowsky stained 
slides.

As a CAP inspector, I have also observed labs who utilize a buckle smear for 
direct smear preps and also for liquid based preps.  They will run them through 
the stains to ensure that they work as expected before staining patient 
specimens. I don't think there is one correct way to perform this evaluation 
but the key is to make sure that you are evaluating each type of prep and the 
stain that you perform in your lab each day.  

Joe W. Walker, Jr. MS, SCT(ASCP) 
Anatomical Pathology Manager
Rutland Regional Medical Center
160 Allen Street, Rutland, VT 05701
P 802.747.1790  F 802.747.6525
joewal...@rrmc.org, www.rrmc.org
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To be the Best Community Healthcare System in New England
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-----Original Message-----
From: Mary Ann via Histonet <histonet@lists.utsouthwestern.edu> 
Sent: Monday, July 16, 2018 12:52 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cap PAP Smears Controls

Hello,

In preparing for CAP I have a question:
Does anyone run a positive PAP control with their run?

Sent from my iPhone

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