In the past, I've used a rapid acetylcholinesterase on frozen material, but we never used Osmium to enhance staining. I've also used IHC on FFPE sections using s-100 and peripherin. Works great, but it's been awhile, so I don't know if there is something better/easier out there now.
Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal ------------------------------ Message: 3 Date: Mon, 13 Aug 2018 19:11:34 +0000 From: "Hobbs, Carl" <carl.ho...@kcl.ac.uk> When I was doing them biopsies ( pre-operative) to establish status, we used a std histochemical method on frozen sections of biopsy material ( visualising acetylcholinesterase activity). It used Osmium to enhance /darken positivity so, NOT recommended these days. I recall somebody leaving the lid off the Osmium ( stored at 4C)?..the fridge plastic lining was BLACK the next day! So, any antibody that identifies nerve fibres and ganglion cells should be equally effective, using IHC/IF? Sure, interpretation is critical ( identifying presence/absence of ganglion cells and "significant" increase in lamina propria of nerve fibres as compensatory mechanism) There must be an internationally recognised/accepted protocol? Which technique does Great Ormond Street Hospital/St Thomas' Hospital use, these days? Curious-illy Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
