I agree with Jamie
Only a few Ags are "oxidised" ( that's the term used, I recall) but, don't let it be YOUR protein of interest. If you really are concerned, cut fresh sections and immunostain along with your stored sections. Imho: cut as few sections as you need. Store any unused at 4C Best wishes Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet