I guess the question is, who told you this and what is their reference? Most special stains don't require a hood. Only those that have volatile chemicals or odors that may be offensive (ie, ammonium sulfide). We have some areas we do specials without a hood. Our main special stain areas have a "comfort" hood that is not a real chemical hood but more like a hood in an oven hood in a commercial kitchen. They are not certified or checked in any way and we do not claim they are safety hoods.
Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Laurie Colbert via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, August 21, 2018 7:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special stains and hoods I would like to know how many people perform (manual) routine special stains under a hood. We do basic specials - Giemsa, AB/PAS, GMS, Trichrome, Muci - but we do them on the counter, not under a hood. We have been told the staining needs to be done under a hood, but I have never done that in my entire career. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet