Histotechs: I am looking a protocol to stain osmium tetroxide....Is anybody can share their thoughts...on a frozen section.
I attached an idea of how this person wants me to follow his protocol with this staining but I kind of lost... my PI request: I'll be perfusing the mice with saline solution 0.9% followed by 10% formalin Cryoprotect the tissue with 30% sucrose Cut 30 micron sections in cryostat and mount on slides I would handle you the slides, since the tissue contains Fluorescent proteins, must be protected from light Once the staining with osmium is finished, you can give me the slides back to check the staining and the fluorescence before it is dehydrated and coverslip. thanks for your help Blanca Lopez HT (ASCP) Histotechnologist UT Southwestern Medical Center Harold C. Simmons Comprehensive Cancer Center Histology Lab K1-210 214-648-7598 blanca.lo...@utsouthwestern.edu<mailto:blanca.lo...@utsouthwestern.edu> ________________________________ UT Southwestern Medical Center The future of medicine, today. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
