Hi
Depends on what you mean by cryosections. Unfixed/fixed? Stored at RT, 4C, -20C, -80C. Stored dry or in glycerol So many variables! My opinion is to store blocks and cut sections as required. Least variables. Sure, one loses some tissue everytime one cuts anew....a good thing. It IS complicated so, a project has to be thought out well in advance. Stimulating post Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet