Hi everyone, Does anyone has experience performing IHC staining for platelets on mouse FFPE?
I've been trying for a while with different primary antibodies unsuccessfully. I found two that work in cryosections, but not a single hint of positivity on FFPE tissues. The samples have been prepared as usual (NBF fixation, graded EtOH, Xylene, wax...) and thin sections were collected by usual procedures (think they are 5 um). Unfortunately I have limited resources, so my protocol has been manual staining (no autostainer) and I have only microwave or water baths available around to play with Ag retrievals. I also already tried Sodium Citrate and Citrate buffers at pH = 6 and 9. Anyone has a protocol that works and that they would be willing to share? Much appreciated! Kind regards, Ana Protecting your privacy is important to us. The Baker Heart and Diabetes Institute will handle your information in accordance with the Privacy Act 1988 (Cth) and its Privacy Policy which is available at www.baker.edu.au or on request by contacting priv...@baker.edu.au or by calling 1800 838 498. The Privacy Policy also explains how you can access and correct your personal information, or make a complaint about a breach of the Australian Privacy Principles. bidipp2014.0.1a -- Message protected by MailGuard: e-mail anti-virus, anti-spam and content filtering.http://www.mailguard.com.au/mg _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
