Hi Reuel, In my experience this is the ORO crystallizing out on the section. I would first filter your staining solution, then keep your staining times to as short as you can. Also changing between 37 and room temperatures may be useful too.
mills ᐧ On Wed, Oct 3, 2018 at 8:39 AM Reuel Cornelia via Histonet < histonet@lists.utsouthwestern.edu> wrote: > I was wondering if you could help me know why our Oil Red O have some > black snowflakes on our fat tissue hours after staining or after 24 hours > more snowlflakes precipitation occurs. The staining was reference was from > Lillie RD, Ashburn. Please note that our staining works well between two to > three hours. Is there a reason fro this precipitation? > > > Reuel > > TSRH > > Dallas, TX > > ---------------------------------------------------------------------- > Texas Scottish Rite Hospital for Children is one of the nation's leading > pediatric centers for the treatment of orthopedic conditions, certain > related neurological disorders and learning disorders, such as dyslexia. > This email transmission and/or its attachments may contain confidential > health information, intended only for the use of the individual or entity > named above. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Biology www.3Scan.com 415 2187297 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet