Our repeat limits differ. If it is due to tissue falling off, we will try at least 3 times If it is due to unexpected, but within control, we will repeat it 2 times Then if it is still problematic for the above 2, we will send out the block for staining. If it is due to technical error (wrong block, wrong stain) we will perform the stain until correct, and it better not be more than once, LOL
Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 1. Histotechs abroad? (Wellen, Terry :LLS Lab) 2. Re: Frozen sections and cold acetone (Hobbs, Carl) 3. REPEATS IN IHC (Kurth Virginia L.) 4. Re: REPEATS IN IHC (Rene J Buesa) Message: 3 Date: Fri, 26 Oct 2018 20:07:22 +0000 From: Kurth Virginia L. <vku...@uwhealth.org> Subject: [Histonet] REPEATS IN IHC Hello, I was wondering if anyone in the histology laboratory (IHC) has a limit on how many times one would repeat a stain; for example but not Limited too: The tissue fell off, negative control, wrong block,etc.. for IHC DAB and/or ISH. Thanks Ginny ------------------------------ Message: 4 Date: Sat, 27 Oct 2018 13:48:06 +0000 (UTC) From: Rene J Buesa <rjbu...@yahoo.com> To: "Kurth Virginia L." <vku...@uwhealth.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] REPEATS IN IHC Message-ID: <1773692175.85882.1540648086...@mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Ginny:It seems you are asking for a "problem" average and that will vary per laboratory and how the whole process is carried out.Section washed out from the slide usually is consequence of poor processing or sectioning and has nothing to do with IHC, although, perhaps, a wrongly performed HIER can cause it.Wrong block is a mistake also having nothing to do with IHC and is always caused by an inattentive HT not paying attention to the process.My point is that, essentially, the lab should try to get to "0" mistakes and any one has to be documented in the QC record but it will be interesting to find out the "mistakes rate" you find and I think you should share it with all.Ren? On Friday, October 26, 2018 4:41 PM, Kurth Virginia L. via Histonet <histonet@lists.utsouthwestern.edu> wrote: Hello, I was wondering if anyone in the histology laboratory (IHC) has a limit on how many times one would repeat a stain; for example but not Limited too:? The tissue fell off, negative control, wrong block,etc.. for IHC DAB and/or ISH.? Thanks Ginny _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet