Hello, I need a counterstain for use together with Sudan Black B. I am using this method: https://www.ncbi.nlm.nih.gov/pubmed/27812872 which uses Nuclear Fast Red. I don't have nuclear fast red... In an updated protocol by these authors they use hematoxylin but in a previous trial I found it was too dark and make visualizing the Sudan Black signal too difficult. I'm using Sigma's Mayer's Hematoxylin solution. It is past its sell-by date and just won't blue nicely any more..
I have available Methyl Green, Neutral Red and Safranin O. The former washes out in aqueous/glycerol mounting medium (required for the protocol). How well does Neutral Red and Safranin work with an aqueous mounting medium? Thanks -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. *Don't panic if I don't reply to your emails sent over the weekend. I don't usually have time to check my email over the weekend and will get back to you on Monday.* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet