A quick question: Are you using any recycled reagents? ie xylene, alcohol

John


www.ciqc.ca

‐‐‐‐‐‐‐ Original Message ‐‐‐‐‐‐‐
On Wednesday, November 14, 2018 12:24 PM, MONICA D. LOCKHART via Histonet 
<histonet@lists.utsouthwestern.edu> wrote:

> Hello Histo Fam!!
>
> Please help!
>
> My facility is experiencing frequent H & E staining problems. Everything from 
> cloudy cells on routine GI to "The Blank Look" on PNB's.
>
> We have made changes in some of the obvious areas like cassette whole size 
> and discontinued the use of sponges at grossing. However, with our biopsies, 
> we still see varying results in our H&E staining.
>
> Though this is not an exhaustive list of all the things we have encountered 
> or the number of changes we have made, hopefully this will give you an idea 
> of what we are seeing and maybe you have ran across this problem and can 
> share your wisdom.
>
> Any comments would be appreciated and most welcome.
>
> Monica D. Lockhart, BBA, HT (ASCP) PBT
> Supervisor Clinical Labs Histology
> Loyola University Medical Center
> 2160 S. First Ave, Bldg 110 Rm 2290
> Maywood, IL 60153
> (o) 708.327.2608
> (c) 708.692.8361
> monica.lockh...@luhs.org
>
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