Gareth, reguarding the eosin biopsy post on the histo net. Things you might want to considered looking at, I cannot say for sure because I don't know your processing solutions/schedule:
Most Eosins are alcohol soluable, by putting the eosin in your formalin most of it will wash out during processing. Many labs put the Eosin in the last alcohol. Hematoxylin is typically water soluable, if you put it in formailin processing usually removes water if the formailin is followed by alcohols. I hope this helps, Cassie From: Gareth Davis via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, 10 January 2019 9:15 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Opinion on dye on biopsies So, I work in a small GI lab, and I put Eosin in my first formalin on my processor. My biopsies are very small and this helps, somewhat, to see the specimens for embedding and cutting. But, unfortunately, the esophagus tissues do not absorb the eosin much. Anyway, the hospital lab I work, part-time, in has started using hematoxylin to help see their biopsies. I happen to embed there and I think it just makes a big mess and the tissue does not absorb much of the stain. What are other labs doing to aid in making their small biopsies easier to see? What are pros and cons to doing this, in your opinion? Thanks! -- *Ms. Gareth B. Davis*, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
