Doesn't sound like a fixation issue to me. Could the tissue be drying out before it's placed in formalin? Also, are these specimens inked for assessment of margins? I've seen ink interfere with immunoreactivity.
Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) richard.car...@hhchealth.org -----Original Message----- From: Debra Siena via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Friday, February 08, 2019 11:51 AM To: 'histonet' Subject: [Histonet] Derm IHC question CAUTION: This email is from outside HHC. USE CARE when opening attachments or links. Hello fellow Histonetters I would like to ask you a question about IHC staining and derm cases. I am seeing a peculiar issue going on, where the melanocytes in the middle of the tissues are staining pretty well but when you get to the ends of the tissues either shaves or ellipses, they are not staining. This is sporadic, not every case and there is no consensus as to a common thread between the cases. I feel that this may be a fixation issue but was just wondering if anyone had ever seen the same phenomena and would be willing to share the theory or even better what was the remedy behind this issue. The fixative is 10% Neutral Buffered Formaliln and the cells in question that are "dropping out" which is what the pathologist is describing are melanocytes, especially with Sox-10 and Mart 1 antibodies. Thanks for the assistance, I definitely appreciate it very much. Best wishes, [image001] Debbie Siena, HT(ASCP)QIHC Empowering Anatomic Pathology Technical Support Manager, StatLab 2090 Commerce| McKinney, TX 75069 t: 800.442.3573 ext 229 | m: 469-400-6897 | f: 972-436-1369 dsi...@statlab.com<mailto:dsi...@statlab.com>|https://urldefense.proofpoint.com/v2/url?u=http-3A__www.statlab.com&d=DwICAg&c=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=niLAvgTbNiAqFJIM-oaLdmyfDgppcOQotF3-vo_Qv1M&s=y7Dkipiv1JdLkqba8XolhtugffjOReKOTWuW0mZaJJI&e=<https://urldefense.proofpoint.com/v2/url?u=http-3A__www.statlab.com_&d=DwICAg&c=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=niLAvgTbNiAqFJIM-oaLdmyfDgppcOQotF3-vo_Qv1M&s=uHeyPcAgHiInkXaGAN29W0BdT9ZKO4f1uuvarGmSNRs&e=> StatLab is an ISO 13485 Certified Company _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=niLAvgTbNiAqFJIM-oaLdmyfDgppcOQotF3-vo_Qv1M&s=9GfWQBzHH2U1LIbnBXO5wvUeCTlJulcFAkXN5aWrbCw&e= Reminder: This e-mail and any attachments are subject to the current HHC email retention policies. Please save or store appropriately in accordance with policy. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
