Cindy McGrady at Hurley Medical Center in Flint, Michigan asks: >>Our laboratory is experimenting with xylene free processing on our Sakura VIP, substituting isopropanol for our xylene substitute. Our pathologists have complained about processing, especially the biopsies, with our regular processing, they have the washed out look, nuclear staining is faint. If you are using the xylene free processing, can you provide feedback, good or bad, any suggestions for optimal processing, etc?<<
If the isopropanol isn't working, you need to go back to your xylene substitute, or if it didn't work, to a different aliphatic xylene substitute - they aren't all alike (and if you're going to recover them by distillation, you'll need to stick with a single brand). - I suppose few people are processing with limonene (smells like citrus fruit) any more. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
