Stainers and coverslippers:Let me interject in the discussion:1- Sakura is
"hands down" my preferred option because of reliability and results
consistency2- Having to have a water free last ethanol is something of the past
(besides just proper protocol) on the other hand for years I have dewaxed my
sections with dishwasher soap and obtained excellent dewaxing without xylene or
ethanol, at all.3- After staining (or any "staining" protocol) I dehydrate any,
and I mean any, "stained" by placing them in a 60ºC oven during 5-6 minute to
completely dry and then to the coverslipper, eliminating, again, ethanol and
xylene. Both these two steps (and those dealing with tissue processing) have
made my laboratory (and several others) absolutely xylene (or xylene
substitutes) free.4- I have always preferred film over glass for speed and
simplicity. No problems with "coverslip peeling" if the amount of delivered
xylene (the only instance when I use xylene) is correct.5- Finally, never ask
for input to a "vendor" for they all have "tainted" opinions at different
levels and will systematically recommend the products they sell. Do your
homewotk and ask users, not sllers.I also hope this will helpRené
On Thursday, March 14, 2019 3:59 PM, Gudrun Lang via Histonet
<histonet@lists.utsouthwestern.edu> wrote:
Hi Terri,
Have you actually used the Spectra from Leica? This is the follower of
ST5020 and CV5020.
I am also interested in some feedback on this instrument.
Gudrun Lang
-----Ursprüngliche Nachricht-----
Von: Terri Braud via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Gesendet: Donnerstag, 14. März 2019 20:10
An: 'histonet@lists.utsouthwestern.edu'
Betreff: [Histonet] Stainer vs. Stainer
Hi Alison -
I've used both stainers and like both of them a lot. Both were super
reliable and easy to use. However, coverslipping is a different story.
I've used both film and glass. About film - super quick, super easy, but -
the purity of the xylene used to coverslip from film must be absolute.
Anyone who has experienced film pulling off the slides in storage had a
miniscule portion of water carried down the acohols and into the xylene. If
it were glass, the process is a bit more forgiving of water contaminent. The
absolute alcohols leading to the end xylenes must be kept very fresh. I
kept film slides for over 20 years, no problem. If you are looking into
digital pathology, I would check with vendors to see if film is acceptable.
I don't know.
As to coverslippers, we've been using the Sakura glass now for 10 years and
love it. I can't compare it to the newer Leica Glass, but 10 years ago my
techs all preferred the Sakura because it had fewer moving parts and the
maintenance was easier. I hope this helps. Good Luck, Terri
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal
6. stainer v. stainer (Perl , Alison)
Message: 6
Date: Wed, 13 Mar 2019 20:08:14 +0000
From: "Perl , Alison" <ap...@cmmedical.com>
To: "'histonet@lists.utsouthwestern.edu'"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] stainer v. stainer
Hi all
We are getting ready to purchase a new H&E stainer/coverslipper, and are
considering the Sakura Prisma Plus (tape) and the Leica Spectra (glass).
Does anyone have good or bad feedback on either instrument, and/or tape v.
glass? We've always had glass, but of course the coverslippers need more
maintenance, take longer to dry, more expensive than tape, etc etc. So we
are very interested in tape, but still a little hesitant about the old
problems of yellowing and peeling after 10+ years. Since we're in NY, we
have to keep all slides for 20 years....
Any thoughts are appreciated!
Alison Perl, HTL(ASCP)CM
Anatomic Pathology Manager
CareMount Medical
110 South Bedford Rd
Mount Kisco, NY 10549
(914) 302-8424
ap...@cmmedical.com<mailto:ap...@cmmedical.com>
www.caremountmedical.com<http://www.caremountmedical.com/>
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