My preference, whenever possible, is to steer people towards using normal tissues for controls in IHC. They are consistent in quality and appearance and fixation and processing can be better controlled. I suggest you use Appendix for Ki67 and evaluate the lymphoid follicles plus there are some positive staining cells in the crypts. Should you feel the need to use tumour and quantify the amount staining you could use Mantle Cell Lymphoma. This should give reasonably accurate consistency of Ki67 count through the block. See the article "Standardization of Positive Controls.........." https://www.researchgate.net/publication/282488454_Standardization_of_Positive_Controls_in_Diagnostic_Immunohistochemistry
I am most interested in "selling" the concept of iCAPCs ( immunohistochemistry Critical Assay Performance Controls) and any feedback is appreciated. John www.ciqc.ca ‐‐‐‐‐‐‐ Original Message ‐‐‐‐‐‐‐ On Monday, April 8, 2019 11:17 AM, Dessoye, Michael via Histonet <histonet@lists.utsouthwestern.edu> wrote: > Hello Histonet! > > Looking for some advice regarding ER/PR/Her2 controls. I'm able to buy > commercially TMA blocks containing varying levels of positivity for ER, PR, > and Her2. This work nicely because you can have a negative and varying levels > of positive control as a same-slide control. But our docs would like to score > Ki-67 as well. Currently, they are just doing pos/neg for Ki67. > > It will take some time to collect cases and create a similar control, so I'm > wondering what y'all are using? Is there anything commercially available? Or > is everybody just collecting cases with varying degrees of positive? > > Thanks, > Mike > > Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor > | Commonwealth Health Laboratory Services | > mjdessoye@commonwealthhealth.netmailto:mjdess...@commonwealthhealth.net | 575 > N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: > 570-552-1484 > > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It is intended > only for the use of the individual(s) and entity named in the message. If you > are not an intended recipient of this message, please notify the sender > immediately and delete the material from your computer. Do not deliver, > distribute or copy this message and do not disclose its contents or take any > action in reliance on the information it contains. > > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
