Hi Charles I've had very good luck in the past with Poly Scientific's Gomori's retic kit, which is ready-to-use (no ammoniacal preparation!) and very reliable. I haven't used AMT's. I wonder if anything changed in the transition to StatLab ownership? Making the ammoniacal generally seems to be the trickiest part...
Alison Perl, HTL(ASCP)CM Anatomic Pathology Manager (914) 302-8424 ap...@cmmedical.com -----Original Message----- From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Monday, April 22, 2019 2:57 PM To: Histo List Subject: [EXTERNAL] [Histonet] Retic staining I am having trouble getting my retic stain to come out strong. It is working but Pathologists are saying they shouldn't have to go to high power just to see it (and I agree). I am using a brand new American MasterTech Chandler's Precision kit. I have followed all the steps correctly (one difficulty is getting the Ammonium hydroxide to go drop by drop so this may be the issue). I have tried staining longer in ammoniacal silver. more time in 20% formalin, more and less time in gold chloride and sodium thiosulfate. If anyone has any suggestions or uses this kit with time modifications please let me know. Willing to try anything to get it working. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and its attachments, if any, may contain confidential or proprietary information and are intended solely for authorized use by the intended recipient(s) only. Any other use of this email is prohibited. If you have received this email in error, you are hereby notified that any retention, disclosure, copying, forwarding, distribution (in whole or in part and whether electronically, written and/or orally) and/or taking of any action in reliance on this email, its contents and/or any attachments thereto is strictly prohibited. If you received this email in error, please notify the sender by replying to this message and permanently delete this email, and any attachments thereto, from your system immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
