Fellow Histonetters - I would appreciate your feedback on an intermittent issue that has shown up in our lab. All of a sudden, we are having difficulty with tissue specimens falling off of our slides on the IHC stains and special stains sporadically. About a year ago, we switched instruments on the IHC bench from the Leica BOND to the ROCHE ULTRA, and we use the Ventana NexES special stainer. No adherence issues with all of the validation slides that were run. We have tried various types of slides recently as well, and the issue still prevails.
Would any of you mind telling me your slide workflow - type of slide used, gelatin or not used in flotation bath, how long slides are cooked, etc.... for your IHC slides, for your special stains slides, and even for your H&E slides. I would welcome your suggestions and feedback. Thank you so much!!! Deanne Knutson Supervisor Anatomic Pathology [X] "Let All Be Received as Christ." ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
