Whoops, The Ventana does not use Victoria blue, my mistake. They have a resorcin fuchsin solution (known as Hart's method) not Miller Victoria blue.
Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Tony Henwood (SCHN) Sent: Saturday, 27 July 2019 10:44 AM To: Bob Richmond <rsrichm...@gmail.com> Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Victoria blue for lung tissue Victoria Blue is the dye used in Miller's Stain for Elastic Tissue. It is also used in the Roche Ventana Benchmark Stainer to stain elastic Tissue: Miller PJ (1971) An elastin stain. Med Lab Technol 28, 148-149 Karen Percival & Zaher Radi (2017) Comparison of five elastin histochemical stains to identify pulmonary small vasculature, Journal of Histotechnology, 40:3, 73-78 Yufeng Yu & Clifford M. Chapman (2000) "Elastic Tissue Staining in Human Skin" Histologic 32(1): 12 Roten SV, Bhat S, Bhawan J. Elastic fibers in scar tissue. J Cutan Pathol 1996: 23: 37-42. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Bob Richmond via Histonet <histonet@lists.utsouthwestern.edu> Sent: Saturday, July 27, 2019 4:10 AM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Victoria blue for lung tissue Amy (where?) asks: >>I was asked to do Victoria Blue stain on rodent FFPE lung tissue to exam thickness of artery. Could anybody recommend a good vendor of this reagent kit?<< You can get Victoria Blue R (Colour Index 44040) from Sigma-Aldrich and several others. I couldn't find anyone who offers a kit, only the dry dye. There are other dyes called Victoria Blue, reported to give the same results. You'd have to find a method for preparing it as an elastic stain, and I couldn't find such a method either with Google or in my old books. The requester needs to supply you with a method. I suspect the requester is reading an old article. There are stains for elastic tissue (which is I suppose what you want to "exam thickness of artery") that are a lot easier to get. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
