Michelle (Shelly) Aono (at Auburn in Alabama) asks: >>Does anyone have any experience changing from HemoDe to VWR Xylene Substitute? Did you have to extend your processing times? By how much?
When people talk to good "recycling" of the clearing agent, does that mean re-use? As in how often you need to discard/refill the reservoirs in the processor? I currently coverslip with Eukitt mounting medium, is this compatible? Anything other advice you can offer or things you have discovered during your switch?<< HemoDe is a limonene clearing agent. I think these are rapidly going out of use. VWR Xylene Substitute is one of a large number of "aliphatic" clearing agents. These are synthetic hydrocarbons, similar to the petroleum cut called naphtha. They are odorless (at least to my insensitive nose), and much less toxic than xylene. They do not all have the same composition, with a wide range of flash points. They are readily recycled, but each one has its own distillation routine (or did with the older generation of recyclers, anyway), so that if you are to recycle, you can't easily change brands, something you need to check with whoever it is in your outfit decides who your vendors are. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
