We cut alot if these. This is the protocol: 1. The microtome, blade and water bath are cleaned well. We follow withRNAse Away wipe down as an added precaution.
2. We use a coplin jar of 100% ethanol for forceps, pick or whatever other tool you might use that comes into contact with and dip and clean in between each sample. I actually have 2 sets and put them into the alcohol and switch out each time. 3 I use the RNAse free tubes to put the curls into. 4. You must wear gloves. 5. If they don't pick the samples up right away they are placed in a -20 freezer for storage. They have good results. Respectfully, Colleen Forster HT(ASCP) U of MN On Sun, Sep 8, 2019, 10:36 AM John Garratt via Histonet < histonet@lists.utsouthwestern.edu> wrote: > The best I can offer is to advise you to wipe the knife holder area down > with a cleaning solvent and use a fresh blade. Clean forceps with a solvent > and place the scroll (50 microns) directly into a labelled 1.5mL plastic > vial. I would be interested on other thoughts on this. > > John > > > > www.ciqc.ca > > > > > ‐‐‐‐‐‐‐ Original Message ‐‐‐‐‐‐‐ > On Friday, September 6, 2019 3:05 PM, Erin McCarthy via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > > > Hello Histonetters, > > > > I am curious if any of you perform scrolling of paraffin embedded tissue > > for molecular studies? If so, what precautions do you take to minimize > > contamination? I am asking specifically about at the cutting station as > > that is my area of expertise. But we are looking to pilot this for > samples > > that in the past have had large quantities of recuts. > > > > Thank you for any information you can provide! > > > > > ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- > > > > Erin McCarthy, HT (ASCP) > > Histology Supervisor > > > > Tempus Labs > > 600 W. Chicago Ave. > > Chicago IL 60654 > > Cell: (708)269-8610 > > > > > ---------------------------------------------------------------------------------------------------------------------- > > > > This email and any attachments may contain privileged and confidential > > information and/or protected health information (PHI) that is protected > by > > federal and state privacy laws. It is intended solely for the use of > > Tempus Labs and the recipient(s) named above. Nothing contained in this > > communication and any attachments thereto is intended to waive any > > privileges or rights of confidentiality. If you are not the recipient, > or > > the employee or agent responsible for delivering this message to the > > intended recipient, you are hereby notified that any review, > dissemination, > > distribution, printing or copying of this email message and/or any > > attachments is strictly prohibited. * If you have received this > > transmission in error, please notify us immediately at (855)-442-8305 > > and permanently delete this email and any attachments*. > > > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet