Perhaps they did an orcein stain with H&E counterstain? That would make the copper associated protein a dark red-brown colour but with other components looking like an H&E.
Penny Marr Senior BMS C/- Histology Conquest Hospital St Leonards-on-Sea TN37 7RD penny.m...@nhs.net (01424) 758023 -----Original Message----- From: Jennifer Phinney [mailto:jh...@vet.k-state.edu] Sent: 10 September 2019 15:57 To: HistoNet Subject: [Histonet] Detecting copper in H&E staining? Hello all, I am in a veterinary histology lab. One of my Pathologists mentioned that at two of the locations he'd been at previously (Ithaca, NY and Saskatoon, Canada) copper deposits stained in the routine H&E slides. Is anyone aware of how this could have been achieved? I assume it would either have to be an additive in the eosin or perhaps something naturally occurring in the tap water rinses? Thanks for any assistance, Jennifer Phinney QIHC Histology Laboratory Administrator KSVDL ******************************************************************************************************************** This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in relation to its contents. To do so is strictly prohibited and may be unlawful. Thank you for your co-operation. NHSmail is the secure email and directory service available for all NHS staff in England and Scotland. NHSmail is approved for exchanging patient data and other sensitive information with NHSmail and other accredited email services. For more information and to find out how you can switch, https://portal.nhs.net/help/joiningnhsmail _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
