Two good screening stains are Mallory and Parker’s Fresh Hematoxylin Stain for Metals and Timm’s Silver Sulphide Method for Metals. Malloy's results: Aluminium Blue-black Copper Greenish-blue Iron Blue-black Lead Blue Zinc Blue
For more specific staining: Aluminon Stain for Aluminium Hydroxide Walton’s Stain for Aluminium (Phloxine binds the aluminium) Bedrick et al (1986) method for Zinc Rubeanic Acid Technique for Copper Rhodanine Technique for Copper These methods are quite sensitive but there are some specificity issues. I can provide further details and references if required. Here are some: Ohtsuki, Y., Yamaguchi, T., Sonobe, H., Takahashi, K., Hayashi, K., Takenaka, A., ... & Terao, N. (1989). Stain Technology: A Simplified Aluminum Stain in Paraffin Sections of Bone from Hemodialysis Patients. Stain technology, 64(2), 55-59. Walton, J. R., Diamond, T. H., Kumar, S., & Murrell, G. A. C. (2007). A sensitive stain for aluminum in undecalcified cancellous bone. Journal of inorganic biochemistry, 101(9), 1285-1290. Bedrick, A. E., Ramaswamy, G., & Tchertkoff, V. (1986). Histochemical determination of copper, zinc, and iron in some benign and malignant tissues. American journal of clinical pathology, 86(5), 637-640. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children’s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP) via Histonet <histonet@lists.utsouthwestern.edu> Sent: Monday, 30 September 2019 20:49 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Metals Morning all! I need some advice re: protocols to demonstrate metals in FFPE tissues. Metals such as copper, aluminum and zinc. Thanks much! Jeanine Sanders, BS, HT(ASCP), QIHC(ASCP) Centers for Diseases Control and Prevention 1600 Clifton Road NE MS H18-SB Bldg. 18, Rm SB-114 Atlanta, GA 30329 404-639-3590 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet