RE:How to prevent bubbling in pressure cooker for immunohistochemistry procedure.
Hi there Mark. Really appreciate your feedback. I do use tween 20 in the retrieval solution but actually thought it was to clean the slides, thus preventing background staining. I did not know it prevents bubble formation, so i have learnt something. However, i use it at a 0.5 % solution. Maybe thats too much as the solution looks a bit soapy, and could also cause lifting of sections im presuming. Thanks again, keep well. Best regards Subash Get Outlook for Android<https://aka.ms/ghei36> ________________________________ From: histonet-requ...@lists.utsouthwestern.edu <histonet-requ...@lists.utsouthwestern.edu> Sent: Friday, October 18, 2019 7:00:01 PM To: histonet@lists.utsouthwestern.edu <histonet@lists.utsouthwestern.edu> Subject: Histonet Digest, Vol 191, Issue 11 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet<https://protect-za.mimecast.com/s/w04kCk5jm7Sm0Q2WTJJF48> or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RELIA HOT JOB ALERT! IHC Specialist for New England area. Contact me for info ASAP! (Pam Barker) 2. How to prevent bubbling in pressure cooker for immunohistochemistry procedure (Donovan, Mark) ---------------------------------------------------------------------- Message: 1 Date: Thu, 17 Oct 2019 14:23:52 -0400 From: "Pam Barker" <rel...@earthlink.net> To: "Histopeeps Histonet" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] RELIA HOT JOB ALERT! IHC Specialist for New England area. Contact me for info ASAP! Message-ID: <01ce01d58518$0759e9a0$160dbce0$@earthlink.net> Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters! I hope you are having a great day and cruising into the weekend. I have an opportunity that I want to share in hopes that you or someone you know might be interested. I am looking for a Field Applications Support Specialist ?IHC for a territory in New England. This is a RELIA EXCLUSIVE! My client offers an excellent product line and reputation to support it. This is a full time salaried position NO sales required. Use your IHC expertise to help my client?s customers further their support of patient care. My client is offering a very competitive salary, travel expenses, benefits and more. If you would like details on the opportunity please contact me ASAP! Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: rel...@earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA<https://protect-za.mimecast.com/s/8sqVClOkn7IgKZRnsYvqB2> www.linkedin.com/in/reliasolutions<https://protect-za.mimecast.com/s/AOdnCmw0oyUEYnyJhNQG0-> www.twitter.com/pamatrelia<https://protect-za.mimecast.com/s/hFsbCnZmpOtMLo9Oh0J3bO> ------------------------------ Message: 2 Date: Thu, 17 Oct 2019 23:10:56 +0000 From: "Donovan, Mark" <m.dono...@alfred.org.au> To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] How to prevent bubbling in pressure cooker for immunohistochemistry procedure Message-ID: <me2pr01mb43226cc8011ccaeaea33cefebc...@me2pr01mb4322.ausprd01.prod.outlook.com> Content-Type: text/plain; charset="us-ascii" Sabash, What I think you may be referring to is the use of a surfactant in the antigen retrieval solution to lower the surface tension. The theory being it reduces bubble formation in the retrieval solution at high temperature and hence disruption of tissue. Before moving to commercial antigen retrieval solutions we would add Tween 20 to a concentration of 0.05% to our in house citrate or EDTA retrieval solutions to achieve this effect. Regards, Mark Original message: Date: Wed, 16 Oct 2019 13:43:28 -0400 From: Maria Cruz <mcruz8...@gmail.com<mailto:mcruz8...@gmail.com>> To: "histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>" <histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>> Subject: Re: [Histonet] How to prevent bubbling in pressure cooker for immunohistochemistry procedure Message-ID: <capg7ww1f0dsbw85m8wnrz2pamxpgkqpxw0v9kx-svxmwbv1...@mail.gmail.com<mailto:capg7ww1f0dsbw85m8wnrz2pamxpgkqpxw0v9kx-svxmwbv1...@mail.gmail.com>> Content-Type: text/plain; charset="UTF-8" Subash: Since the main purpose of using a pressure cooker for HIER is the rapid and reliable pre-treatment of sections while PREVENTING boiling and potential tissue detachment, you really shouldn?t be experiencing any bubbling. If you are, then your PC isn?t sealed adequately and the only way to fix that problem is to replace the gasket or find another way to tighten the lid.. I?ve never heard of anything that you can put inside of a PC that can change the way it pressurizes, so I?ll be following this thread to see if anyone else can contribute. Maria ************************************** Hi there all Can anybody please tell me if they have heard of or perhaps know of a substance that can be placed in the pressure cooker during the antigen retrieval process for immunohistochemistry. This prevents excessive bubbling and lifting up of sections. I did somehow come across such info a while back, but seem to have lost it. Many thanks. Best Regards Subash Govender ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet<https://protect-za.mimecast.com/s/w04kCk5jm7Sm0Q2WTJJF48> ------------------------------ End of Histonet Digest, Vol 191, Issue 11 ***************************************** Disclaimer - University of Cape Town This email is subject to UCT policies and email disclaimer published on our website at http://www.uct.ac.za/main/email-disclaimer or obtainable from +27 21 650 9111. If this email is not related to the business of UCT, it is sent by the sender in an individual capacity. Please report security incidents or abuse via https://csirt.uct.ac.za/page/report-an-incident.php. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
