In our lab, we evaluate a slide from a case in each staining run and document 
the staining of the nucleus and cytoplasm that are present within the slide.  
We also run a blank slide in each batch and evaluate it for any potential 
floaters.  Lastly, we stain our FNA specimens separately from any other non-gyn 
or FNA case.  We are CAP accredited these steps have fulfilled their 
requirements.

Joe W. Walker, Jr. MS, SCT(ASCP)
Anatomical Pathology and Interim Phlebotomy Manager
P 802.747.1790
joewal...@rrmc.org

-----Original Message-----
From: Olszewski, Dawn via Histonet <histonet@lists.utsouthwestern.edu>
Sent: Monday, November 4, 2019 9:11 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cyto Controls for PAP

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Good afternoon everyone,

I am now the manager over Histology and Cytology.  I know very little about 
Cytology, so I am hoping you guys can help me out.  We do non-gyn cyto but do 
not have a cytotechnologist.  Our pathologist read all the slides and the histo 
techs do the prep work.  We are probably going to CAP soon and one of the 
questions is, "how do you assess the quality of cytopathology stains"? For 
histology we run a H&E control down the stain line prior to staining any 
patient slides and evaluate it for proper staining of the nucleus and the 
cytoplasm.  How is this handled in cytology using the PAP stain?  If you make 
controls, how is it done and stored and what criteria do you use to assess the 
quality?  Sorry for my ignorance, but I am a histotech and not a cytotech by 
trade.



Dawn Olszewski HTL(ASCP)QIHC

Pathology Manager

South Georgia Medical Center

P: (229) 259-4830

E: dawn.olszew...@sgmc.org

Dawn Olszewski
Pathology Manager - Histotechnologist
Laboratory

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South Georgia Medical Center
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