If you have access to spare fresh tissue (ie placenta) you can infuse the tissue with fungus (blue cheese if you wish) and cultivate overnight before FFPE, I have found these make excellent control blocks and the tissue makes for good structural support, plus the tissue architecture might make the blue cheese more palatable to pathologists. Your idea is certainly not crackers.
John www.ciqc.ca ‐‐‐‐‐‐‐ Original Message ‐‐‐‐‐‐‐ On Friday, November 22, 2019 5:01 PM, Akemi via Histonet <histonet@lists.utsouthwestern.edu> wrote: > Hi Histopeeps! Here’s my procedure for Fungus Test Controls since we are out > of controls. This was from Blue Cheese I processed yesterday. It turned out > fabulous!!! Photo credit to Christina George (i know Histonet doesn’t attach > photos so can send to you) and lab credit to my staff, Quinnlan Dewitt and > Carlos Duran. I used President brand Le Blue which I purchased from Sprouts > grocery. It cost $7.95 for 3.5 oz. it was the most aged. I then took a > portion of the “moldy blue” cheese and put it in lens paper, then cassetted > it and fixed it in 10% Formalin in the morning for 8 hours and ran it > normally with routine surgicals. Thinking of publishing! > > Cheers! > > Sent from my iPhone > > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
