I’ve done it a handful of times when my only Histotech called in sick. No effect on quality.
Garrey Sent from my iPhone > On Dec 18, 2019, at 2:24 AM, Jennifer Saunders via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > I don’t see a problem with it. We all melt and re-embed tissue for all kinds > of reasons every day. I think it is much better for the tissue than keeping > it warm for an extended period of time. Jen Saunders > > > Sent from Yahoo Mail for iPhone > > > On Tuesday, December 17, 2019, 2:28 PM, Tony Henwood (SCHN) via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > Hi Paula, > > We routinely do this, especially for our fetal autopsy blocks. > We are then able to process and let them set at room temp until we are able > to embed and cut. > Some cases are more urgent than others so these can be expedited a lot easier > since they will only need embedding, sectioning and staining. We also get > best usage of our limited processing capabilities. > It is more efficient for us. > > Morphology, staining and immunohistochemistry is not affected. > > It is better to do this rather than leave them at 64oC plus for extended > times prior to embedding since many antigens will be adversely affected. > > Take home point - do not overcook your tissues. > > > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children’s Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > -----Original Message----- > From: Theresa Dalton via Histonet [mailto:histonet@lists.utsouthwestern.edu] > Sent: Wednesday, 18 December 2019 7:34 AM > To: P Sicurello <pat...@gmail.com> > Cc: HistoNet <histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Delay in embedding? > > We have done this - only on an emergency basis. > > Sent from my iPhone > >> On Dec 17, 2019, at 2:48 PM, P Sicurello via Histonet >> <histonet@lists.utsouthwestern.edu> wrote: >> >> Good Morning Listers, >> >> >> >> How many out there will process tissue and then leave the cassettes at >> room temperature and embed it at a later time (hours or the next day)? >> >> >> >> Please send me your opinions about doing this. I think it’s a bad >> idea, others I speak with disagree. >> >> Sincerely, >> >> Paula Sicurello, HTL (ASCP)CM >> >> Histotechnology Specialist >> >> UC San Diego Health >> >> 9300 Campus Point Drive >> >> La Jolla, CA 92037 >> (P): 858-249-5610 >> >> >> >> *Confidentiality Notice*: The information transmitted in this e-mail >> is intended only for the person or entity to which it is addressed and >> may contain confidential and/or privileged material. Any review, >> retransmission, dissemination or other use of or taking of any action >> in reliance upon this information by persons or entities other than >> the intended recipient is prohibited. If you received this e-mail in >> error, please contact the sender and delete the material from any computer. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message is intended for the addressee named and may contain confidential > information. If you are not the intended recipient, please delete it and > notify the sender. > > Views expressed in this message are those of the individual sender, and are > not necessarily the views of NSW Health or any of its entities. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
