We do it all the time.
Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal -----Original Message----- From: histonet-requ...@lists.utsouthwestern.edu [mailto:histonet-requ...@lists.utsouthwestern.edu] Sent: Wednesday, December 18, 2019 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 193, Issue 12 CAUTION: This is an EXTERNAL EMAIL. Stop and think before clicking links or opening attachments. Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Delay in embedding? (P Sicurello) 2. Re: Delay in embedding? (Theresa Dalton) 3. Re: Delay in embedding? (Tony Henwood (SCHN)) 4. Re: Delay in embedding? (Jennifer Saunders) 5. Re: Delay in embedding? (Garrey Faller) 6. Happy Holidays Histonetters! Here are the 12 days of Christmas for histotechs by Ashley Troutman. Enjoy! (Pam Barker) 7. plural fluid prep (Michelle Jamison) ---------------------------------------------------------------------- Message: 1 Date: Tue, 17 Dec 2019 11:30:58 -0800 From: P Sicurello <pat...@gmail.com> To: HistoNet <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Delay in embedding? Message-ID: <CAPSjddacHtoHxwmgHzqY_jF+1r=SExBHUwV0uK=ui3zf0_1...@mail.gmail.com> Content-Type: text/plain; charset="UTF-8" Good Morning Listers, How many out there will process tissue and then leave the cassettes at room temperature and embed it at a later time (hours or the next day)? Please send me your opinions about doing this. I think it?s a bad idea, others I speak with disagree. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. ------------------------------ Message: 2 Date: Tue, 17 Dec 2019 15:33:43 -0500 From: Theresa Dalton <terrie.dal...@icloud.com> To: P Sicurello <pat...@gmail.com> Cc: HistoNet <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Delay in embedding? Message-ID: <693b4d62-df63-486c-9017-d24b9f190...@icloud.com> Content-Type: text/plain; charset=utf-8 We have done this - only on an emergency basis. Sent from my iPhone > On Dec 17, 2019, at 2:48 PM, P Sicurello via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > ?Good Morning Listers, > > > > How many out there will process tissue and then leave the cassettes at room > temperature and embed it at a later time (hours or the next day)? > > > > Please send me your opinions about doing this. I think it?s a bad idea, > others I speak with disagree. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 9300 Campus Point Drive > > La Jolla, CA 92037 > (P): 858-249-5610 > > > > *Confidentiality Notice*: The information transmitted in this e-mail is > intended only for the person or entity to which it is addressed and may > contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this e-mail in error, > please contact the sender and delete the material from any computer. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Tue, 17 Dec 2019 22:28:13 +0000 From: "Tony Henwood (SCHN)" <tony.henw...@health.nsw.gov.au> To: Theresa Dalton <terrie.dal...@icloud.com>, P Sicurello <pat...@gmail.com> Cc: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Delay in embedding? Message-ID: <e97666e5e1ab4aef946b83e65f8b4...@svdcmbx-mex024.nswhealth.net> Content-Type: text/plain; charset="utf-8" Hi Paula, We routinely do this, especially for our fetal autopsy blocks. We are then able to process and let them set at room temp until we are able to embed and cut. Some cases are more urgent than others so these can be expedited a lot easier since they will only need embedding, sectioning and staining. We also get best usage of our limited processing capabilities. It is more efficient for us. Morphology, staining and immunohistochemistry is not affected. It is better to do this rather than leave them at 64oC plus for extended times prior to embedding since many antigens will be adversely affected. Take home point - do not overcook your tissues. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Theresa Dalton via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, 18 December 2019 7:34 AM To: P Sicurello <pat...@gmail.com> Cc: HistoNet <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Delay in embedding? We have done this - only on an emergency basis. Sent from my iPhone > On Dec 17, 2019, at 2:48 PM, P Sicurello via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > ?Good Morning Listers, > > > > How many out there will process tissue and then leave the cassettes at > room temperature and embed it at a later time (hours or the next day)? > > > > Please send me your opinions about doing this. I think it?s a bad > idea, others I speak with disagree. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 9300 Campus Point Drive > > La Jolla, CA 92037 > (P): 858-249-5610 > > > > *Confidentiality Notice*: The information transmitted in this e-mail > is intended only for the person or entity to which it is addressed and > may contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action > in reliance upon this information by persons or entities other than > the intended recipient is prohibited. If you received this e-mail in > error, please contact the sender and delete the material from any computer. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ------------------------------ Message: 4 Date: Wed, 18 Dec 2019 07:07:35 +0000 (UTC) From: Jennifer Saunders <jsaunder...@yahoo.com> To: "Tony Henwood (SCHN)" <tony.henw...@health.nsw.gov.au>, Theresa Dalton <terrie.dal...@icloud.com>, P Sicurello <pat...@gmail.com> Cc: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Delay in embedding? Message-ID: <920732173.736881.1576652855...@mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 I don?t see a problem with it. We all melt and re-embed tissue for all kinds of reasons every day. I think it is much better for the tissue than keeping it warm for an extended period of time.?Jen Saunders? Sent from Yahoo Mail for iPhone On Tuesday, December 17, 2019, 2:28 PM, Tony Henwood (SCHN) via Histonet <histonet@lists.utsouthwestern.edu> wrote: Hi Paula, We routinely do this, especially for our fetal autopsy blocks. We are then able to process and let them set at room temp until we are able to embed and cut. Some cases are more urgent than others so these can be expedited a lot easier since they will only need embedding, sectioning and staining. We also get best usage of our limited processing capabilities. It is more efficient for us. Morphology, staining and immunohistochemistry is not affected. It is better to do this rather than leave them at 64oC plus for extended times prior to embedding since many antigens will be adversely affected. Take home point - do not overcook your tissues. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Theresa Dalton via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, 18 December 2019 7:34 AM To: P Sicurello <pat...@gmail.com> Cc: HistoNet <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Delay in embedding? We have done this - only on an emergency basis. Sent from my iPhone > On Dec 17, 2019, at 2:48 PM, P Sicurello via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > ?Good Morning Listers, > > > > How many out there will process tissue and then leave the cassettes at > room temperature and embed it at a later time (hours or the next day)? > > > > Please send me your opinions about doing this.? I think it?s a bad > idea, others I speak with disagree. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 9300 Campus Point Drive > > La Jolla, CA 92037 > (P): 858-249-5610 > > > > *Confidentiality Notice*: The information transmitted in this e-mail > is intended only for the person or entity to which it is addressed and > may contain confidential and/or privileged material.? Any review, > retransmission, dissemination or other use of or taking of any action > in reliance upon this information by persons or entities other than > the intended recipient is prohibited.? If you received this e-mail in > error, please contact the sender and delete the material from any computer. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Wed, 18 Dec 2019 05:09:23 -0500 From: Garrey Faller <garr...@gmail.com> To: Jennifer Saunders <jsaunder...@yahoo.com> Cc: "Tony Henwood (SCHN)" <tony.henw...@health.nsw.gov.au>, Theresa Dalton <terrie.dal...@icloud.com>, P Sicurello <pat...@gmail.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Delay in embedding? Message-ID: <31c3bb02-24d4-4c02-aba5-c744b4fe7...@gmail.com> Content-Type: text/plain; charset=utf-8 I?ve done it a handful of times when my only Histotech called in sick. No effect on quality. Garrey Sent from my iPhone > On Dec 18, 2019, at 2:24 AM, Jennifer Saunders via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > ?I don?t see a problem with it. We all melt and re-embed tissue for all kinds > of reasons every day. I think it is much better for the tissue than keeping > it warm for an extended period of time. Jen Saunders > > > Sent from Yahoo Mail for iPhone > > > On Tuesday, December 17, 2019, 2:28 PM, Tony Henwood (SCHN) via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > Hi Paula, > > We routinely do this, especially for our fetal autopsy blocks. > We are then able to process and let them set at room temp until we are able > to embed and cut. > Some cases are more urgent than others so these can be expedited a lot easier > since they will only need embedding, sectioning and staining. We also get > best usage of our limited processing capabilities. > It is more efficient for us. > > Morphology, staining and immunohistochemistry is not affected. > > It is better to do this rather than leave them at 64oC plus for extended > times prior to embedding since many antigens will be adversely affected. > > Take home point - do not overcook your tissues. > > > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children?s Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > -----Original Message----- > From: Theresa Dalton via Histonet [mailto:histonet@lists.utsouthwestern.edu] > Sent: Wednesday, 18 December 2019 7:34 AM > To: P Sicurello <pat...@gmail.com> > Cc: HistoNet <histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Delay in embedding? > > We have done this - only on an emergency basis. > > Sent from my iPhone > >> On Dec 17, 2019, at 2:48 PM, P Sicurello via Histonet >> <histonet@lists.utsouthwestern.edu> wrote: >> >> ?Good Morning Listers, >> >> >> >> How many out there will process tissue and then leave the cassettes at >> room temperature and embed it at a later time (hours or the next day)? >> >> >> >> Please send me your opinions about doing this. I think it?s a bad >> idea, others I speak with disagree. >> >> Sincerely, >> >> Paula Sicurello, HTL (ASCP)CM >> >> Histotechnology Specialist >> >> UC San Diego Health >> >> 9300 Campus Point Drive >> >> La Jolla, CA 92037 >> (P): 858-249-5610 >> >> >> >> *Confidentiality Notice*: The information transmitted in this e-mail >> is intended only for the person or entity to which it is addressed and >> may contain confidential and/or privileged material. Any review, >> retransmission, dissemination or other use of or taking of any action >> in reliance upon this information by persons or entities other than >> the intended recipient is prohibited. If you received this e-mail in >> error, please contact the sender and delete the material from any computer. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message is intended for the addressee named and may contain confidential > information. If you are not the intended recipient, please delete it and > notify the sender. > > Views expressed in this message are those of the individual sender, and are > not necessarily the views of NSW Health or any of its entities. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Wed, 18 Dec 2019 11:32:13 -0500 From: "Pam Barker" <rel...@earthlink.net> To: "Histopeeps Histonet" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Happy Holidays Histonetters! Here are the 12 days of Christmas for histotechs by Ashley Troutman. Enjoy! Message-ID: <0a5001d5b5c0$b402ac40$1c0804c0$@earthlink.net> Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters, Happy Holidays! Here is a gem posted on the histonet in 12/2013 The 12 days of Christmas for Histotechs ! written by Ashley Troutman ?Hello all, I hope the holidays are treating everyone well. (Especially in light of us having the most hazardous job in the country...) Here's some fun for the season: The Twelve Days of Histo TWELVE Medical Directors ELEVEN logs-a-printing TEN residents reading NINE frozen sections EIGHT administrators SEVEN stainers beeping SIX techs complaining FIVE I...H...Cs.... :) FOUR calling docs THREE recuts TWO special stains And a block with an H and EEEEE... Please feel free to sing along. Merry Christmas in Histoland!? Ashley Troutman BS, MBA, HT(ASCP) QIHC Histonetters, I have some REALLY Exciting opportunities, Here?s a quick list: Leadership: MA - Field Applications Specialist ? IHC- Boston IL - Field Applications Specialist ? IHC ? Chicago MO - Histology Lab Manager ? St. Louis TN - Histology Supervisor - Nashville Histotechnicians/Histotechnologists North Carolina South Carolina Wisconsin Florida Kansas New York California I have new opportunities coming in all of the time. Let me know if anything looks interesting on my list or if there is a specific area you would like for me to be on the lookout for. We can start chatting now about opportunities after the new year!! The early bird gets the worm!! I also can help if you are short staffed! I can be reached asap on my cell/text at 407-353-5070 or email me at rel...@earthlink.net Happy Holidays to you and yours!!!! Happy Holidays !!!! Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: rel...@earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ------------------------------ Message: 7 Date: Wed, 18 Dec 2019 10:51:40 -0700 From: Michelle Jamison <m.jami...@elitechgroup.com> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] plural fluid prep Message-ID: <d4756630-95cf-220b-00bb-d9c9ab50b...@elitechgroup.com> Content-Type: text/plain; charset=utf-8; format=flowed Hi All, I work in a research lab where we are using plural fluid to proof a method. Recently we have received some, and are unsure how long it will be ok in the fridge, or if we should add some preservation fluid to it. Can anyone advise please. Thank you in advance, and Happy Holidays to all!! -- Best of all things to you, Michelle Jamison // Tel : +1.435.752.6011 Ext. 435.227.1474 m.jami...@elitechgroup.com <mailto:> ? www.elitechgroup.com <http://www.elitechgroup.com/> ------------------------------------------------------------------------ Logo ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 193, Issue 12 ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet