As a student in the 1960s I was told by my elders and betters to filter all staining solutions before using. It was very good advice. Filtration does not prolong the life of a stain, but it does remove crud, a material that can be composed of polymerized dyes and bits of previously stained cells or tissues.
Fifty years on, I can identify a few staining solutions that never deteriorate into insoluble materials that fall out of solution even after 10+ years. Even for these, filtration before use is good laboratory practice. Not filtering any stain may therefore be bad laboratory practice. Happy New Year, John Kiernan = = = ________________________________ From: Haas, Elizabeth via Histonet <histonet@lists.utsouthwestern.edu> Sent: 31 December 2019 11:46 To: S hay <susanha...@gmail.com> Cc: histonet@lists.utsouthwestern.edu <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Pap stains I believe filtering stains daily is a CAP requirement Sent from my iPhone > On Dec 31, 2019, at 9:23 AM, S hay via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > 1. Does everyone filter their pap stains daily? > 2. Are you chaining all other reagents daily? > > Thanks in advance. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ LEGAL DISCLAIMER: This message and all attachments may be confidential or protected by privilege. If you are not the intended recipient you are hereby notified that any disclosure, copying, distribution, or use of the information contained in or attached to this message is strictly prohibited. Please notify the sender of the delivery error by replying to this message and then delete it from your system. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
