I agree with Carl about the X-gal activity being destroyed by processing
for paraffin sections (I'm a researcher, not a histologist).
However, in our hands the anti-b-galactosidase antibody, reference
A-11132, from ThermoFisher DOES work on paraffin sections (many others
don't). It has been published with subsequent incubation with a
biotinylated secondary (Vector BA-1000) and standard procedures
thereafter to show good signal with DAB. We do immunofluorescence.
MPBio used to sell a good polyclonal for this purpose, but it has since
been discontinued.
Best wishes,
Heather
--
*Marseille Medical Genetics Centre
<https://www.marseille-medical-genetics.org/fr/organisation/>*
INSERM logo <https://www.inserm.fr/>
AMU logo <https://www.univ-amu.fr/>
*Heather C. Etchevers, Ph.D.*
*MMG - INSERM U1251*
Faculté de Médecine AMU
27 boulevard Jean Moulin
13005 Marseille - France
Tel: +33(0)4 91 32 49 37
heather.etchev...@inserm.fr <mailto:heather.etchev...@inserm.fr>
CREST-NET groupement de recherche
CNRS GDR 2031
*CREST-NET*
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