Terri, I agree with John on the use of Histogel. I use it routinely for all the cell blocks and tiny matrix samples that come down and I get excellent results.
I do a lot of IHC and the results are good. Respectfully, Colleen On Thu, Jan 23, 2020 at 11:51 AM John Garratt via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi Terri, I suggest you use Histogel for block preparation. It works > exceptionally well, it is good for IHC and does not have the pitfalls of > plasma/thrombin. > Plasma/thrombin does work well for cell blocks but you will have to > consider an ethical and safe source for your plasma. > The instructions for using Histogel are in the package insert though I > have one comment. Be careful how you warm the Histogel and use a heat > block. Do NOT use a microwave since there is a tendency to overheat the gel > and you will end up with poor quality IHC. > > John > > > www.cpqa.ca > > ‐‐‐‐‐‐‐ Original Message ‐‐‐‐‐‐‐ > On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > > > Hi fellow Histonetters - I'm in need of some help, please > > Background - We currently use agar to capture our scant cell blocks for > processing. I am unfamiliar with the Plasma/Thrombin method of cell block > preparation and am interested in comparing it to our current method > > Request - Could you please send me your procedures for this method, > specifically where you purchase your plasma and thrombin and what species > are used? > > Thanks in advance. Histotechs rock! > > > > Terri L. Braud, HT(ASCP) > > Anatomic Pathology Supervisor > > Laboratory > > Holy Redeemer Hospital > > 1648 Huntingdon Pike > > Meadowbrook, PA 19046 > > ph: 215-938-3689 > > fax: 215-938-3874 > > Care, Comfort, and Heal > > > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory B173 PWB 612-626-1930 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet