Q has been answered: fix in "10% Formalin" made up from concentrated liquid which has 10% Alcohol to retard reformation of paraformaldehyde polymers. Once the 40% liquid has been diluted to 4% the fixative effects of alcohol are minimised. Alcohol does not dissolve glycogen ( otherwise the dehydration stage of Pwax processing would) The Main Man JK has OK'd this. I am glad that he still posts/passes on his extensive knowledge! I can see further by having his shoulders to stand on.... Glycogen is still demonstrable ( with PAS) but, as JK pointed out, the Formalin will "push" the glycogen up to the plasma membrane as it fixes the proteins, giving what I learnt to call "streaming" artefact In 2014, I posted an image in Histonet Images of glycogen streaming in a FFPWS skin section stained with PAS. Have a look?
Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
