This is a very difficult target to detect. I was told recently by a vendor that one needs to use prolonged, very aggressive antigen retrieval (pressure cooker with high pH buffer) to get consistent results. Also, the type of cancer may influence your success (colon vs. thyroid vs. melanoma). I would be interested in knowing what others' experiences are.
Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) richard.car...@hhchealth.org -----Original Message----- From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Friday, March 27, 2020 8:07 AM To: Histo List Subject: [Histonet] BRAF testing This is an email from an external source. USE CAUTION opening attachments or links from unknown senders. Can anyone send me a block that can be used to validate BRAF V600E antibody. I have tried several cases that should work but our pathologists don't think it is working correctly. I just need a small core sample that is known to be positive. Any help would greatly be appreciated -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KCs9X-8!Oq9k97gGgRatvdlIoqX-QkrcMRMS19Iuxle4pZY1FqKfIdEBOVk2YOJ3yVO0qAn3MBg$ Reminder: This e-mail and any attachments are subject to the current HHC email retention policies. Please save or store appropriately in accordance with policy. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
