Fix in 10% NBF - 10 minutes Rinse in running tap water - 5 minute Rinse in distilled water Gill 2 Hematoxylin - 1 1/2 minutes 1% Glacial Acetic water - 3 dips Running water - 2 minutes (we do not need bluing agent - our water takes care of it) 95% Ethanol -2 minutes 1% Eosin/Phloxine - 2 minutes 95% Ethanol - 2 minutes Remaining dehydration in absolute ethanol & xylene - 2 mintues rest of the way down
________________________________ From: Raible, John C. via Histonet <histonet@lists.utsouthwestern.edu> Sent: Friday, April 24, 2020 8:10 AM To: histonet@lists.utsouthwestern.edu <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Routine H&E frozen section protocol Hello Histonet, My name is John Raible, and I work at the VA medical center in Minneapolis. I am trying to find routine H&E frozen section staining protocols for an automated linear stainer. My lab recently got one, and I was asked to research staining protocols Any help you could provide would be greatly appreciated. Thank you all. Stay safe and healthy! John _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!jZVhszUj4q_tZtfO2cKIo_OQbyOsjHXT6pEGAn_UXytcWKRzM703NRorymJh6A$ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet