Isopropanol processing is routinely used with formalin fixation. Isopropanol is miscible in paraffin, so you can skip the clearing agents. Just run it down to 100% Isopropanol as you would do on a Ethanol processing schedule, then straight into paraffin. Check this out:
https://pubmed.ncbi.nlm.nih.gov/14534624/ 😊 Tracy Tracy Pheneger, HT/HTL (ASCP), QIHC (ASCP), LATg (AALAS) Senior Professional Research Assistant BioServe Space Technologies University of Colorado c/o Kolicko AES Univ of Colorado Rm 224, 3775 Discovery Dr Boulder CO 80309 From: Betsy Molinari via Histonet<mailto:histonet@lists.utsouthwestern.edu> Sent: Wednesday, June 3, 2020 12:50 PM To: Porter, Amy<mailto:port...@msu.edu>; 'Histonet@lists.utsouthwestern.edu'<mailto:histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] stored in 80% isopropanol I am also in research. I have very little to go on, but I presume it is not going to yield good results. Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: bmolin...@texasheart.org texasheart.org<https://www.texasheart.org/> | facebook<https://www.facebook.com/Texas.Heart.Institute> | twitter<https://twitter.com/Texas_Heart> From: Porter, Amy <port...@msu.edu> Sent: Wednesday, June 3, 2020 1:47 PM To: Betsy Molinari <bmolin...@texasheart.org> Subject: Re: stored in 80% isopropanol *** Important *** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. ________________________________ Rubbing alcohol - potentially really dehydrated already depending on how long??? I am research so no recommendation for clinical..... ________________________________ From: Betsy Molinari via Histonet <histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>> Sent: Wednesday, June 3, 2020 2:39 PM To: 'Histonet@lists.utsouthwestern.edu' <Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu>> Subject: [Histonet] stored in 80% isopropanol Hi, If tissue is fixed in formalin ( I do not know if it is 10% NBF) then put in 80 % isopropanol can the tissue be processed as if it was in ETOH? What are the effects to the tissue stored in isopropanol? Thanks, Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: bmolin...@texasheart.org<mailto:bmolin...@texasheart.org> texasheart.org<https://urldefense.com/v3/__https://www.texasheart.org/__;!!HXCxUKc!g0oZkH9IjlZnl595iDir-L42QNjnDXR4fAz-6J8E2Nnlq46NtE0EQZaVQLaVZA$ <https://urldefense.com/v3/__https:/www.texasheart.org/__;!!HXCxUKc!g0oZkH9IjlZnl595iDir-L42QNjnDXR4fAz-6J8E2Nnlq46NtE0EQZaVQLaVZA$%20> > | facebook<https://urldefense.com/v3/__https://www.facebook.com/Texas.Heart.Institute__;!!HXCxUKc!g0oZkH9IjlZnl595iDir-L42QNjnDXR4fAz-6J8E2Nnlq46NtE0EQZYhiwnDeg$ <https://urldefense.com/v3/__https:/www.facebook.com/Texas.Heart.Institute__;!!HXCxUKc!g0oZkH9IjlZnl595iDir-L42QNjnDXR4fAz-6J8E2Nnlq46NtE0EQZYhiwnDeg$%20> > | twitter<https://urldefense.com/v3/__https://twitter.com/Texas_Heart__;!!HXCxUKc!g0oZkH9IjlZnl595iDir-L42QNjnDXR4fAz-6J8E2Nnlq46NtE0EQZYfRwq3Dw$ <https://urldefense.com/v3/__https:/twitter.com/Texas_Heart__;!!HXCxUKc!g0oZkH9IjlZnl595iDir-L42QNjnDXR4fAz-6J8E2Nnlq46NtE0EQZYfRwq3Dw$%20> > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!g0oZkH9IjlZnl595iDir-L42QNjnDXR4fAz-6J8E2Nnlq46NtE0EQZYYGhZaig$<https://urldefense.com/v3/__http:/lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!g0oZkH9IjlZnl595iDir-L42QNjnDXR4fAz-6J8E2Nnlq46NtE0EQZYYGhZaig$<https://urldefense.com/v3/__http:/lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!g0oZkH9IjlZnl595iDir-L42QNjnDXR4fAz-6J8E2Nnlq46NtE0EQZYYGhZaig$%3chttps:/urldefense.com/v3/__http:/lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!g0oZkH9IjlZnl595iDir-L42QNjnDXR4fAz-6J8E2Nnlq46NtE0EQZYYGhZaig$>> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
