As a research lab, we sometimes would like to use paraformaldehyde-fixed but non-paraffin embedded tissues; paraffin embedding alters antigens and necessitates antigen retrieval, but simple fixation does not. We have done the traditional 30% sucrose before OCT and freezing, with cryostat sectioning, but results are inconsistent, sometimes producing Swiss-cheese brains. Does anybody have an alternative to 30% sucrose that is more reliable? I didn’t see anything in the Archives after a search for “30% sucrose”. Thanks very much, Ed Roy
Edward J. Roy, PhD Professor Emeritus Department of Molecular and Integrative Physiology University of Illinois at Urbana-Champaign Urbana, IL 61801 217 333-3375 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
