Original Message----- From: Ken M via Histonet <histonet@lists.utsouthwestern.edu> Sent: Tuesday, July 28, 2020 11:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Apple Green Birefringence in Amyliod slides
Hi Ken, May I give you my personal experience on Congo Red? 1) Always cut thick tissue (ie,10um thickness, but 8 um will work) 2) Always fresh cut tissue. You can pre-cut control slides, but store them in the *dark* @ *4C (ie. fridge, never RT)*. Amyloid will fade if pre-cut and stored at room temperature with light. We always stored our stained control slide as reference along with our pre-cut unstained slides in the fridge, but "apple green birefringence" will also fade with time (unstable). *Madeleine Huey, **B.S., HTL & QIHC (ASCP)* Supervisor, Pathology/Histology & IPOX (MV & LG) El Camino Hospital 2500 Grant Road (Rm GC-33) Mountain View, CA 94040 Phone: 650-940-7038 Fax: 650-988-8387 Email: madelein...@elcaminohospital.org Hi everyone. I was wondering if anyone out there has any experience with diagnosing Amyloid tissue using Congo Red stained Kidney using polarized lenses. Is it common to use polarized light to detect Amyloid deposits? Does the absence of the "apple green birefringence" indicate a problem with the control tissue or the control slides? Should this green bifringence always appear to confirm the diagnosis? I know that the tissue should be cut thicker than normal (we usually cut at 5), but in the future maybe we will cut at 7 or 8? On Thu, Jul 30, 2020 at 10:04 AM <histonet-requ...@lists.utsouthwestern.edu> wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Apple Green Birefringence in Amyliod slides (John Kiernan) > 2. Sakura Tissue-Tek versus Tanner embedding station Comparison > (Rob Rankin) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 29 Jul 2020 18:39:31 +0000 > From: John Kiernan <jkier...@uwo.ca> > To: Ken M <kdea...@hotmail.com>, "Morken, Timothy" > <timothy.mor...@ucsf.edu> > Cc: Histonet <histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Apple Green Birefringence in Amyliod slides > Message-ID: > < > ytopr0101mb161161fc59817a6b556ccdefa5...@ytopr0101mb1611.canprd01.prod.outlook.com > > > > Content-Type: text/plain; charset="iso-8859-1" > > For another source of polarizing filters, go to a 3D movie, take home the > glasses they provide, and poke out the lenses. They work very nicely as > polarizer and analyzer with an ordinary microscope. > John Kiernan > Anatomy & Cell Biology > University of Western Ontario > London, Canada > = = = > ________________________________ > From: Morken, Timothy via Histonet <histonet@lists.utsouthwestern.edu> > Sent: 28 July 2020 13:57 > To: Ken M <kdea...@hotmail.com> > Cc: Histonet <histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Apple Green Birefringence in Amyliod slides > > Ken, Yes, polarized light and apple green birefringence is diagnostic for > amyloid with congo red and is the best practice. If you have a problem with > known control slides there are two possibilities: 1) make up fresh > solution. The pH has to be right. Or 2) try other control slides. Maybe you > cut through the amyloid area. > > Because we have hundreds of microscopes in our department most just use > polarized film as the polarizer (put over the light source) and another put > over the top of the slide as the analyzer. Turn one of the polarizing > slides and you will see the birefringence appear. > > Source: > "Polarizing film, 2"" x 2"" , PK/10 (BEST For use as a microscope > polarizer)" Cat# S07372 Thermo Fisher Sci Health $36.75 > PK/10 "2" x 2" > > These are polarized film mounted in 2" film holders (like the old > Kodachrome slides). > > Cheap and effective. (and avoids consternation from people losing > expensive microscope polarizers) > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > > -----Original Message----- > From: Ken M via Histonet <histonet@lists.utsouthwestern.edu> > Sent: Tuesday, July 28, 2020 11:43 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Apple Green Birefringence in Amyliod slides > > Hi everyone. I was wondering if anyone out there has any experience with > diagnosing Amyloid tissue using Congo Red stained Kidney using polarized > lenses. Is it common to use polarized light to detect Amyloid deposits? > Does the absence of the "apple green birefringence" indicate a problem with > the control tissue or the control slides? Should this green bifringence > always appear to confirm the diagnosis? I know that the tissue should be > cut thicker than normal (we usually cut at 5), but in the future maybe we > will cut at 7 or 8? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=2jEHe8Hf3ieiSbv1r-ZtSy-mm4FVj1XtTmUSGcfJmE8&s=Q_PpmT_KF2fDUvt9ltFVZLp6ctjM3xkK0RsfuUYW73c&e= > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 2 > Date: Thu, 30 Jul 2020 10:26:57 -0400 > From: Rob Rankin <robran...@rankinbiomed.com> > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Sakura Tissue-Tek versus Tanner embedding station > Comparison > Message-ID: > <CAOEuZ=w1MRj4biRoW9a2muA9oDvWnw4T= > wmhh-kh51dvhst...@mail.gmail.com> > Content-Type: text/plain; charset="UTF-8" > > Is anyone using a Tanner embedding station? If so, what has your experience > been like? I'm trying to compare between a Sakura Tissue-Tek and Tanner > (the Tanner is much cheaper). > > Respectfully, > *Rob Rankin, MSM, SM(ASCP)* > Founder & CEO > Office: 248 625-4104 ext 0012 > www.rankinbiomed.com > > <https://www.rankinbiomed.com/product-categories/> > > 14515 Mackey Rd. > > Holly, MI 48442 > > <https://www.linkedin.com/company/rankin-biomedical-corporation/> > <https://www.youtube.com/channel/UCcieIkDf2_EOHee0PyV8J8Q/videos> > > > *Have not I commanded thee? Be strong and of good courage; be not > afraid, neither be thou dismayed: for the LORD thy God is with thee > whithersoever thou goest. * > > *Joshua 1:9* > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 200, Issue 19 > ***************************************** > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
